Peptide Calculator

Volume (mL) = Desired dose (mg) ÷ Concentration (mg/mL)

If you added 2mL of BAC water to a 5mg semaglutide vial, your concentration is 2.5mg/mL. A 0.25mg dose = 0.25 ÷ 2.5 = 0.1mL = 10 units on an insulin syringe.

The calculator does this arithmetic for you — just enter your vial mg, water volume, and desired dose.

Nothing is ruined — you've just created a lower concentration, which means a larger injection volume to hit your target dose.

Update the BAC water field in the calculator and it will recalculate automatically. The peptide is unaffected — dilution changes the volume per dose, not the compound.

Insulin syringes (0.5mL or 1mL, 29–31 gauge) are standard for subcutaneous peptide injections.

They're marked in "units" where 100 units = 1mL. This calculator shows both unit and mL measurements.

Accurate dosing affects both safety and efficacy. Under-dosing may produce no effect; over-dosing increases side effect risk.

GLP-1 agonists in particular require precise titration to minimise gastrointestinal side effects during dose escalation.

Multiply milligrams (mg) by 1,000 to get micrograms (mcg).

GLP-1 peptides (semaglutide, tirzepatide) are dosed in mg (0.25–15mg). Most other peptides (BPC-157, TB-500, GHK-Cu) are dosed in mcg (250–500mcg) — which is why GLP-1 vials are larger (5–30mg) while others are smaller (5mg = 5,000mcg).

GLOW is a three-peptide blend (GHK-Cu, BPC-157, TB-4) targeting skin rejuvenation — collagen synthesis, elasticity, scar reduction, and hair growth.

KLOW adds KPV, an anti-inflammatory tripeptide that blocks the cell's master inflammatory switch. Choose KLOW for reactive skin, rosacea, or post-procedure recovery.

See the GLOW & KLOW protocol guide for full dosing and cycle details.

GLOW: GHK-Cu 50mg / BPC-157 10mg / TB-4 10mg. GHK-Cu dominates because it drives collagen gene expression in skin.

KLOW: same base plus KPV 10mg.

These ratios are designed for skincare, not injury recovery. See our GLOW/KLOW ratios guide for the corrected injury protocol.

Add 3mL for 25mg/mL. At the standard 0.15mL dose that gives roughly 20 doses per vial.

In the calculator, select GLOW from the dropdown, then update the Cocktail Composition panel to match your vial's exact mg breakdown before entering your BAC water volume.

Add 3mL for roughly 28mg/mL.

Enter all four component values (GHK-Cu / BPC-157 / TB-4 / KPV) in the Cocktail Composition panel before calculating. The ratio-lock feature keeps all four peptides scaling together as you adjust the dose slider.

Click the GLOW / KLOW dropdown and select a preset — GLOW 5/10/30, GLOW 10/10/50, GLOW 10/10/70, KLOW 10/10/10/35, or KLOW 10/10/10/50.

The calculator sets the cocktail composition and locks the dose sliders so all components scale proportionally. You can also unlock the ratios to dose each peptide independently, or use "Custom Cocktail" to enter any composition.

Start low and titrate slowly — rushing is the most common cause of nausea and side effects.

Use our GLP-1 dosing tool to simulate titration schedules and plasma levels for each compound before you start.

Typical dose range: 0.25mg/week (start) → 0.5mg → 1mg → 2–2.4mg (maintenance).

5mg vial: add 2mL for 2.5mg/mL (0.25mg = 10 units, 1mg = 40 units). The 2mL option gives the roundest numbers across the full titration range.

10mg vial: add 2mL for 5mg/mL (0.5mg = 10 units, 1mg = 20 units, 2mg = 40 units).

Typical dose range: 2.5mg/week (start) → 5mg → 7.5mg → 10mg → 12.5mg → 15mg (max).

15mg vial: 3mL for 5mg/mL (2.5mg = 50 units, 5mg = 100 units) or 1.5mL for 10mg/mL (2.5mg = 25 units, 5mg = 50 units). The 10mg/mL option keeps all doses within a single 1mL syringe as you titrate up.

20mg vial: 2mL for 10mg/mL or 4mL for 5mg/mL.

Typical dose range: 1–2mg/week (start) → 4mg → 8mg → up to 12mg (max studied).

12mg vial: 3mL for 4mg/mL (1mg = 25 units, 2mg = 50 units, 4mg = 100 units) — the cleanest unit math across early-to-mid titration. Doses above 8mg require more than 1mL per injection regardless of concentration.

24mg vial: 6mL for 4mg/mL.

Typical dose: 0.15mL (15 units) daily during activation (weeks 1–8), then 2–3x weekly for maintenance.

Add 3mL to a standard 75mg GLOW vial (~25mg/mL) or 85mg KLOW vial (~28mg/mL). At 0.15mL per dose that gives roughly 20 doses per vial.

In the calculator, update the Cocktail Composition panel to match your vial's exact mg breakdown before entering the water volume. Note: GHK-Cu solution appears blue after reconstitution — this is normal.

Add 2mL for a 5mg/mL concentration — the cleanest numbers for both peptides.

BPC-157 — typical dose 250–500mcg/day: 250mcg = 5 units, 500mcg = 10 units.

TB-500 — typical dose 3–5mg twice weekly (loading), 1–2mg weekly (maintenance): 2.5mg = 50 units, 5mg = 100 units.

For a 5mg vial, add 1mL for the same 5mg/mL concentration.

Typical dose: 100–250mg per injection. Start at the lower end — NAD+ can cause significant flushing and discomfort, especially at higher doses.

Add 5mL for 100mg/mL (100mg = 1mL = 100 units) or 10mL for 50mg/mL. Most people use 100mg/mL since common doses stay under 3mL.

Important: Use saline BAC water (bacteriostatic water with sodium chloride) — NAD+ is naturally acidic and standard BAC water causes significant injection site burning. Saline buffers the pH substantially. Inject slowly.

Typical dose — Semax: 200–600mcg/day (N-Acetyl Semax Amidate: 100–300mcg). Selank: 250–500mcg/day (N-Acetyl Selank Amidate: 150–300mcg).

Use bacteriostatic saline (0.9% NaCl with preservative), not standard BAC water — benzyl alcohol alone can irritate nasal mucosa. A standard working concentration is 1mg/mL (0.1%): add 10mL to a 10mg vial. A typical nasal pump delivers ~100mcg per spray at this concentration.

N-Acetyl Amidated versions (NA-Semax Amidate, NA-Selank Amidate) have significantly increased stability and bioavailability — the same concentration reconstitution applies, but the effective dose per spray is higher, so you need fewer sprays to reach your target.

Typical dose — Semax: 200–500mcg SubQ per administration. Selank: 250–500mcg SubQ per administration.

Add 2mL to a 10mg vial for 5mg/mL (5,000mcg/mL): 250mcg = 5 units, 500mcg = 10 units. For a 5mg vial, add 1mL for the same concentration.

N-Acetyl Amidated versions (NA-Semax Amidate, NA-Selank Amidate) offer increased stability and bioavailability due to protective modifications at both ends of the peptide chain. This means a lower dose achieves the same effect — typically 100–200mcg rather than 250–500mcg. Reconstitute the same way, but adjust your target dose in the calculator accordingly.

Add bacteriostatic water slowly down the inside wall of the vial to minimize foaming. Swirl gently — never shake.

The solution should be clear and colorless. See our step-by-step reconstitution guide for visual instructions.

Sterile water containing 0.9% benzyl alcohol, which prevents bacterial growth.

It's the standard diluent for peptides because it keeps the solution stable for weeks rather than hours after the vial is opened.

Most peptides are stable for 28 days refrigerated at 2–8°C. Mark your vial with the reconstitution date.

Discard if the solution becomes cloudy, develops particles, or if you notice reduced efficacy.

No — use separate syringes. Draw BAC water with one syringe, inject into the vial, swirl to mix, then use a fresh syringe to draw your dose.

Reusing the same syringe risks contaminating the vial and dulling the needle before injection. See our reconstitution guide for full technique.